Description
Next-generation sequencing data in BAM and related formats can be processed with a Broad Institute tool suite. These command-line tools are useful for bioinformatics pipelines that mark duplicates, manipulate read groups, validate files, and prepare sequencing data. They are run from a terminal with the picard command and subcommands. Genomic data can be sensitive, so input paths, sample identifiers, logs, and shared outputs need privacy review.